malA [2019-05-30 17:50:17]

6-phospho-alpha-glucosidase

locus: BSU08180

pI: 4.74

mw: 50.35 kDa

protein length: 449 aa

gene length: 1350 bp

function: maltose utilization

product: 6-phospho-alpha-glucosidase

essential: no

ec: 3.2.1.122

synonyms: glvG, glvA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.22|Utilization of maltose]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 890,022 → 891,371

The protein

Catalyzed reaction/ biological activity

• Maltose 6'-phosphate + H₂O = D-glucose + D-glucose 6-phosphate (according to Swiss-Prot)

Protein family

• glycosyl hydrolase 4 family (according to Swiss-Prot)

Structure

• [PDB|1U8X]

[SW|Localization]

• membrane associated [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|C650160C9E5B5F8587CA9E80A57BCCE97D701037|malA]-[gene|AD72489DA260F07B97A213F1F0FBA7CED9471817|glvR]-[gene|E6C7733A12AF9A5322EED444E4A4FF1605A8B20F|malP]
  description: [Pubmed|11489864]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11489864], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|AD72489DA260F07B97A213F1F0FBA7CED9471817|GlvR]: activation, [Pubmed|11489864], in [regulon|AD72489DA260F07B97A213F1F0FBA7CED9471817|GlvR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|11489864], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • induction by maltose ([protein|search|GlvR]) [Pubmed|11489864]

Biological materials

Mutant

• MGNA-C289 (glvA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2287 NBRP B. subtilis, Japan]
• BKE08180 (Δ[gene|C650160C9E5B5F8587CA9E80A57BCCE97D701037|malA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGACGACCTCCTTGAT, downstream forward: _UP4_TAAATGAAATTCCCCCATTT
• BKK08180 (Δ[gene|C650160C9E5B5F8587CA9E80A57BCCE97D701037|malA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGACGACCTCCTTGAT, downstream forward: _UP4_TAAATGAAATTCCCCCATTT

References

• 22900538,16707683,10627040,18763711,9765262,11489864,17676871,15341727,20618987